Tekinoloje Yam'badwo Wotsatira Ikuthamangitsa Chilengedwe Cha Mouse Model
TurboMice ™ Technology
Yopangidwa ndi MingCeler kudzera pakukhathamiritsa kokwanira kwa tetraploid.
Mwa kuphatikizaTekinoloje ya Precise Gene Editingndi mbewa wokometsedwaUkadaulo wa Embryonic Stem Cell Preparation, tsopano titha kusintha pafupifupi jini iliyonse yomwe tikufuna.
TurboMice ™ Technology
Tekinoloje yathu yothandiza kwambiri ya Tetraploid Complementation yatilola kuti tiwonjezere kwambiri kuchuluka kwa mbewa kuchokera ku 1% -5% mpaka 30% -60% ndi maselo opangidwa ndi jini, pafupifupi kufanana ndi kuthekera kwa kusamutsa mwana wosabadwayo.
MingCeler ndi kampani yoyamba padziko lapansi kukwaniritsa bwino kusintha kwaukadaulo wa Tetraploid Complementation kuchokera ku labotale kupita kumakampani.
Traditional Technology
Ukadaulo wachitsanzo wa nyama, monga pronuclear microinjection ndi ES targeting, umakhudza kuswana osachepera 2 mpaka 3 mibadwo kuti apeze mbewa za homozygous genotype, zomwe nthawi zambiri zimatenga miyezi 6-8.
Njira yayitaliyi imalepheretsa kwambiri kupita patsogolo ndi luso la chitukuko chatsopano cha mankhwala ndi kafukufuku wa matenda.
Gulu Lofulumira
1. mpaka 20 homozygous gene-edited mbewa mkati2-4 miyezi.
2. ≈ 50 mbewa homozygous mkati zotsatirazi2 miyezi.
3. 400+ mbewa za homozygous mkati8-12 miyezi.
Lipoti la Nkhani mu 2020
Tekinoloje ya TurboMice ™ idagwiritsidwa ntchito kuti ipange mwachangu500 homozygousmbewa za ACE2 zaumunthu mkati mwa basi8 miyeziza maphunziro a mankhwala ndi katemera wa COVID-19.
Flexible Strain Selection
Njira zachikhalidwe zimakhala ndi malire pakusankha kwamtundu wa mbewa, pomwe ukadaulo wa TurboMice™ umapereka kusinthasintha kochulukirapo ndi mitundu yosiyanasiyana yamitundu yosiyanasiyana yomwe mungasankhe (kuphatikiza Balb /c, ICR, C57BL/6, ndi zina).
● Makoswe amtundu wa F0 opangidwa pogwiritsa ntchito ukadaulo wa TurboMice™ ndi omwe amakhala ndi selo limodzi.
● Choncho, chibadwa cha mbewa za F0 ndizofanana, kuchepetsa zolakwika zoyesera zomwe zimachitika chifukwa cha kusiyana kwa majini.
● Chifukwa chake, deta yoyesera yowunikira mphamvu ya mankhwala ndi zoyeserera zina ndizokhazikika komanso zodalirika.
Amasunga Umphumphu Wabwino Wachibadwa
Mu Situ Precision Gene Editing
● Kusintha kwa majini kwanthawi zonse motsatira milingo ya jini yolondola komanso makulidwe ake enieni.
● Makoswe a ACE2 opangidwa mwaumunthu opangidwa ndi njira zachikhalidwe amapangidwa ndi kutsogoza kwa K18-ACE2, komwe sikungathe kukwanitsa kusintha chifukwa chongoyika mwachisawawa, zomwe zimapangitsa kuti pakhale kusowa kwa mawonekedwe amtundu wa ACE2 mumtundu wopangidwa ndi munthu wa ACE2. .
● MingCeler's ACE2 mbewa mbewa zimaonetsa maonekedwe ake m'ziwalo zosiyanasiyana (zojambula C ndi D pamwambapa), ndipo zimatsanzira bwino zachipatala kutsatira matenda a SARS-CoV-2.
Unique EnhancerPlus Platform
●Pulatifomu ya MingCeler ya EnhancerPlus ikhoza kuthandiza makasitomala athu kukweza mawonekedwe amtundu wamunthu.
● Zotsatira za zaka zambiri za kusonkhanitsa deta mu epigenetics ndi kafukufuku wotsimikizira kuti tsogolo lawo lidzachitike, MingCeler's EnhancerPlus nsanja imatha kuneneratu molondola malo amtundu wa Enhancers, kupanga mapangidwe a njira zosinthira majini kukhala umboni.
● Zotsatira zake, majini omwe akufunidwawo amatha kufanana bwino ndi momwe amafotokozera komanso kuchuluka kwa majini otsalawo.
Lipoti lamilandu: Humanization ya X jini.
● Pogwiritsa ntchito nsanja ya EnhancerPlus, ndondomekoyi inakonzedwa bwino ndipo mlingo wa mawuwo unawona kuwonjezeka kwa maulamuliro atatu (onani pamwambapa graphic), kufika pa zofunikira pa chitukuko cha mankhwala monga momwe makasitomala amachitira pa mlingo wa mapuloteni.
● Atamaliza kupanga mbewa za ACE2 padziko lonse lapansi mu 2020, MingCeler adakweza mtundu wa mbewa wa ACE2 kudzera mumayendedwe anayi kudzera kukhathamiritsa kwa EnhancerPlus, ndipo mulingo wa ACE2 wopangidwa ndi munthu umafika pafupi ndi mawonekedwe a mbewa ACE2.
Mawonekedwe amunthu a ACE2 m'zigawo zosiyanasiyana zamitundu yopangidwa ndi anthu
Multi-Locus Gene Editing
● Ukadaulo wa TurboMice™ ndiye chisankho chabwino kwambiri pamsika popanga mbewa zophatikizika zamitundu yosiyanasiyana, zopatsa ubwino wolondola kwambiri poika malo, kuyika zidutswa zazitali, ndi malo osinthidwa ma genetic angapo, popanda zovuta zosiyanitsa.
● Ukadaulo wa TurboMice™ umalola kusintha munthawi imodzi mpaka ma jini atatu kuti apange ma mozygous multi-locus gene-edited mbewa molunjika kuchokera ku maselo amtundu wa embryonic stem cell, popanda kufunikira kwa nthawi yayitali yobereketsa/kuwunika, kupereka zosiyanasiyana. zamitundu yamtengo wapatali yofunikira pakufufuza kwatsopano kwamankhwala.
● MingCeler apanga bwino mitundu ya mbewa zosinthidwa ma gene mu nthawi yochepa, kutengera mbewa za ACE2.
Kusintha kwamtundu wautali wa Fragment Gene
Ukadaulo wa TurboMice™ umathandizira kusintha kwamitundu yolondola yazidutswa zazitali zopitilira 20kb, kumathandizira kupanga mwachangu mitundu yovuta monga humanization, conditional knock-out (CKO), ndi big fragment knock-in (KI).
Zofalitsa
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin imamanga ACE2 kuti itseke kugwirizana kwake ndi SARS-CoV-2 spike protein ndipo imakhala yothandiza poletsa matenda a SARS-CoV-2 pazitsanzo za nyama.Ma cell Res.2021 Jan; 31 (1): 17-24.doi: 10.1038/s41422-020-00450-0.(IF: 20.507)
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. M'badwo wofulumira wa ACE2 mtundu wa mbewa wopangidwa ndi munthu wa COVID-19 wokhala ndi tetraploid complementation.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.( NGATI: 16.693 )
Kuyenda kwa Utumiki
Zomwe muyenera kuchita ndikutipatsa zomwe mukufuna kusintha jini pamtundu wanu wa mbewa.Kutengera zomwe mwatsimikiza, tidzakhazikitsa dongosolo loyambira, ndipo potsatira kukambitsirana kwina ndi mgwirizano, tisayina mgwirizano waukadaulo.Pambuyo poyambitsa polojekiti tidzapereka zosintha panthawi yake.