Ma cell opangidwa ndi ma gene
Maselo opangidwa ndi ma gene amatanthauza ma cell omwe asintha ma genetic kudzera munjira yomwe imadziwika kuti gene editing.Kusintha kwa majini kumaphatikizapo kusintha DNA ya chamoyo, nthawi zambiri ndi cholinga chowerenga majini kapena kumvetsetsa njira zina zamoyo.
Pogwiritsa ntchito ukadaulo wosinthira ma jini, ndizotheka kukwaniritsa mizere yoyambitsa ma jini, mizere yotulutsa ma cell, kusintha kwa ma point, kapena kugogoda kwa ma cell a ma jini m'maselo enaake, omwe amatha kugwiritsidwa ntchito pophunzira momwe ma jini amagwirira ntchito, ma signature, njira zamatenda. , ndi chitukuko cha mankhwala, komanso kulemba maselo enieni.
Pali zifukwa zingapo zomwe ma cell osinthidwa ndi majini ali ofunikira pakufufuza kwasayansi.Choyamba, amapereka chida chophunzirira momwe majini amagwirira ntchito.Mwa kusintha majini m’malo olamuliridwa, asayansi amatha kuona mmene kusintha kwa majini kumakhudzira khalidwe la maselo kapena njira zina zamoyo.Chidziwitsochi chingathandize kumvetsetsa bwino matenda a majini, komanso kuzindikira zomwe zingatheke kuti zitheke.
Kuphatikiza apo, mizere yosinthidwa ndi majini imatha kugwiritsidwa ntchito kupanga ndi kuyesa mankhwala atsopano.Poyambitsa masinthidwe enieni a majini, asayansi amatha kupanga ma cell omwe amatengera momwe matenda amakhalira, zomwe zimawatheketsa kuwunika momwe machiritso amathandizira.Izi zimathandiza njira zolondola komanso zogwira mtima za chitukuko cha mankhwala.
Kuphatikiza apo, ma cell osinthidwa ndi ma gene amatha kugwiritsidwa ntchito mumankhwala obwezeretsanso.Posintha ma jini omwe amachititsa kuti maselo azikhala kapena kusiyanitsa, asayansi amatha kupititsa patsogolo kuchiritsa kwa ma cell stem kapena kupanga ma cell omwe ndi oyenera kuwaika.
Ubwino
1. Gene-edited multilocus nthawi imodzi;
2. Kupereka chithandizo choyimitsidwa kuchokera ku sgRNA kupanga ndi kaphatikizidwe mpaka kuwunika kwa ma cell;
3. Kudziwa zambiri pakupanga ma gene kuti athandizire kusintha ma gene kuchokera pakupanga dongosolo mpaka kumaliza ntchito yomanga.
Bizinesi Yaikulu
- Gene Knock-out Cell Lines
- Gene Activation Cell Lines
- Lozani mizere yosinthira ma cell
- Knock-in Cell Lines
Maumboni
[1] Zhang S, Shen J, Li D, Cheng Y. Njira zoperekera Cas9ribonucleoprotein kwa CRISPR/Cas9 genome editing.Theranostics.2021 Jan 1; 11 (2): 614-648.doi: 10.7150/thno.47007.PMID: 33391496;PMCID: PMC7738854.